phCMV1/Xi Restriction Sites #sites -- Bp position of recognition site -- AatII 4 3508, 3561, 3644, 3830 Acc65I 1 17 AccI 2 11, 4209 AciI 61 (not expanded) AflII 1 293 AflIII 1 3329 AluI 14 189, 356, 613, 908 1196, 1516, 1974, 2771 3028, 3074, 3164, 3952 4228, 4234 Alw44I 1 3015 AlwI 14 32, 33, 1271, 1582 1661, 1828, 2205, 2220 2579, 2592, 2677, 2689 2763, 4029 AlwNI 2 2507, 2915 ApaI 1 25 ApoI 5 1, 225, 329, 340 4240 AvaI 2 28, 4224 AvaII 3 1927, 2372, 4024 BamHI 1 32 BanI 6 17, 564, 1410, 1445 3852, 4200 BanII 6 25, 598, 1772, 2482 3951, 4227 BbeI 1 1410 BbsI 1 4014 BbvI 13 187, 706, 1371, 1472 1514, 1555, 1648, 2035 2488, 2714, 2920, 2923 2988 BciVI 3 829, 1619, 3120 BfaI 7 66, 260, 678, 1234 2249, 2583, 2836 BglI 5 1180, 3471, 3593, 3664 4035 BglII 1 4220 BlnI 2 1233, 2248 BpmI 3 2209, 2230, 3979 BsaAI 3 525, 1713, 3725 BsaBI 2 69, 1266 BsaHI 8 1410, 2112, 2191, 3508 3561, 3644, 3830, 3984 BsaI 1 2406 BsaJI 19 21, 28, 945, 1017 1140, 1175, 1184, 1233 1574, 1843, 2248, 2375 2376, 2478, 2479, 3169 3747, 4039, 4183 BsaWI 4 1442, 2286, 2976, 3123 BseMII 6 891, 1193, 2074, 2523 2646, 3055 BseRI 1 1214 BseSI 4 25, 1353, 1446, 3015 BsiEI 4 55, 1317, 2992, 4042 BsiHKAI 5 1520, 1710, 3015, 3951 4227 BslI 20 417, 743, 1135, 1386 1930, 2211, 2243, 2244 2375, 2410, 2460, 2461 2492, 2845, 3124, 3290 3308, 3430, 4039, 4178 BsmAI 7 841, 1259, 2278, 2406 3816, 3982, 4165 BsmBI 1 3982 BsmFI 11 942, 1014, 1078, 1548 2080, 2189, 2373, 3548 3699, 3867, 4023 BsmI 2 156, 255 Bsp1286I 11 25, 598, 1353, 1446 1520, 1710, 1772, 2482 3015, 3951, 4227 BspHI 2 837, 2609 BspMI 3 1307, 1670, 2120 BsrBI 4 669, 833, 2022, 2076 BsrDI 1 1637 BsrI 8 440, 1118, 1350, 1551 2797, 2914, 2927, 3682 BssKI 22 28, 29, 39, 946 1001, 1018, 1414, 1574 1797, 2187, 2376, 2479 2951, 3169, 3182, 3303 3476, 3669, 3978, 4021 4045, 4183 BssSI 3 2003, 2129, 3156 Bst4CI 17 15, 500, 1789, 1905 2013, 2336, 2827, 3297 3368, 3461, 3583, 3654 3928, 3966, 4051, 4172 4203 BstAPI 3 972, 1044, 2506 BstBI 3 2092, 4237, 4243 BstDSI 5 21, 1140, 1843, 3747 4039 BstF5I 6 1098, 1265, 1722, 1747 2201, 3989 BstUI 18 22, 59, 334, 710 730, 754, 785, 1476 1777, 2215, 2299, 2362 2431, 2704, 3285, 3446 4040, 4065 Bsu36I 2 890, 2522 Cac8I 29 23, 628, 671, 685 958, 977, 1030, 1049 1303, 1489, 1708, 1774 1780, 1808, 1812, 1853 1857, 1911, 2194, 2381 2428, 2484, 2506, 2717 3277, 3314, 3471, 3664 4197 Cfr10I 4 628, 1730, 1911, 2194 ClaI 1 1250 Csp6I 10 18, 43, 1716, 3605 3630, 3685, 3718, 3769 3926, 4095 CviRI 20 7, 159, 186, 254 971, 980, 1043, 1052 1167, 1243, 1302, 1461 1636, 1650, 2720, 3016 3383, 4055, 4137, 4196 CviTI 78 (not expanded) DdeI 6 891, 1193, 2074, 2523 2646, 3055 DpnI 21 33, 69, 1249, 1253 1272, 1583, 1661, 1742 1751, 1829, 2205, 2221 2580, 2592, 2670, 2678 2689, 2764, 3973, 4029 4221 DraI 3 111, 2553, 2572 DraIII 1 522 DrdI 3 476, 1432, 3221 EaeI 6 55, 1317, 1491, 1882 1909, 4042 EarI 3 883, 1760, 1970 EciI 9 895, 1092, 1113, 1123 1134, 1924, 3111, 3257 3893 Ecl136II 2 3951, 4227 Eco52I 3 55, 1317, 4042 Eco57I 3 1536, 1968, 2802 EcoO109I 1 2508 EcoRI 2 1, 4240 EcoRII 13 946, 1001, 1018, 1797 2376, 2479, 3169, 3182 3303, 3476, 3669, 3978 4183 EheI 1 1410 FauI 14 23, 671, 725, 1080 1101, 1541, 2078, 2216 2297, 2363, 2429, 3472 3498, 3665 Fnu4HI 31 54, 57, 187, 706 728, 742, 1180, 1319 1371, 1382, 1472, 1477 1514, 1555, 1642, 1645 1648, 1884, 1980, 2021 2035, 2149, 2488, 2503 2714, 2920, 2923, 2988 3131, 3286, 4041 FokI 6 1098, 1265, 1722, 1747 2201, 3989 FspI 1 1511 HaeII 4 674, 682, 1410, 3085 HaeIII 22 26, 56, 376, 518 1173, 1179, 1188, 1231 1318, 1492, 1883, 1910 2419, 2476, 2509, 2854 3288, 3306, 3317, 3470 3663, 4043 HgaI 7 755, 2112, 2191, 2650 3228, 3904, 3984 HhaI 20 675, 683, 709, 731 740, 753, 784, 1403 1411, 1475, 1512, 1778 2038, 2214, 2300, 2703 2812, 2986, 3086, 3153 HincII 3 11, 172, 4209 HindIII 1 4233 HinfI 15 62, 454, 476, 886 1896, 2030, 2082, 2140 2177, 2959, 3355, 3797 4069, 4107, 4152 HinP1I 20 675, 683, 709, 731 740, 753, 784, 1403 1411, 1475, 1512, 1778 2038, 2214, 2300, 2703 2812, 2986, 3086, 3153 HpaI 1 172 HphI 5 536, 1597, 2470, 2586 3751 KasI 1 1410 KpnI 1 17 MaeII 15 471, 483, 526, 636 1527, 1714, 2495, 2628 3509, 3521, 3562, 3645 3726, 3831, 4089 MaeIII 13 200, 701, 713, 1532 1838, 2528, 2794, 2910 2973, 3449, 3536, 3885 4086 MboII 10 655, 883, 1760, 1970 2070, 2237, 2437, 2589 2680, 4014 MnlI 26 97, 120, 129, 562 893, 1171, 1177, 1184 1190, 1214, 1217, 1229 1269, 1333, 1469, 1826 2002, 2208, 2256, 2522 2892, 3159, 3216, 3935 4006, 4036 MscI 1 1491 MslI 2 1844, 3748 MspA1I 6 21, 907, 1515, 2744 2989, 4039 MspI 20 29, 39, 629, 1316 1393, 1415, 1443, 1574 1664, 1731, 1912, 2187 2195, 2287, 2761, 2951 2977, 3124, 4021, 4045 MunI 1 161 MvaI 13 946, 1001, 1018, 1797 2376, 2479, 3169, 3182 3303, 3476, 3669, 3978 4183 MwoI 30 632, 662, 689, 731 733, 972, 1044, 1095 1174, 1180, 1396, 1480 1503, 1642, 1648, 1765 1848, 2115, 2302, 2506 2705, 3277, 3471, 3593 3625, 3664, 3757, 3781 3987, 4035 NaeI 3 628, 1911, 2194 NarI 1 1410 NciI 9 28, 29, 39, 1414 1574, 2187, 2951, 4021 4045 NcoI 3 1140, 1843, 3747 NdeI 1 3620 NgoMIV 3 628, 1911, 2194 NlaIII 16 838, 978, 1050, 1141 1282, 1627, 1813, 1844 1870, 2226, 2610, 3330 3381, 3688, 3748, 4139 NlaIV 19 17, 25, 32, 564 585, 597, 788, 949 1021, 1410, 1445, 2293 2372, 2417, 3260, 3299 3852, 4023, 4200 NotI 1 54 NsiI 4 979, 1051, 3382, 4136 NspI 4 977, 1049, 1812, 3329 PciI 1 3329 PleI 9 62, 454, 476, 886 2082, 2959, 3797, 4107 4152 Ppu10I 4 979, 1051, 3382, 4136 PsiI 2 192, 397 PspOMI 1 25 PstI 2 6, 4195 PvuII 2 907, 1515 RsaI 10 18, 43, 1716, 3605 3630, 3685, 3718, 3769 3926, 4095 RsrII 1 1926 SacI 2 3951, 4227 SacII 2 21, 4039 SalI 2 11, 4209 SapI 2 1760, 1970 Sau3AI 21 33, 69, 1249, 1253 1272, 1583, 1661, 1742 1751, 1829, 2205, 2221 2580, 2592, 2670, 2678 2689, 2764, 3973, 4029 4221 Sau96I 11 25, 26, 518, 1927 2372, 2418, 2476, 2509 3470, 3663, 4024 SchI 9 62, 454, 476, 886 2082, 2959, 3797, 4107 4152 ScrFI 22 28, 29, 39, 946 1001, 1018, 1414, 1574 1797, 2187, 2376, 2479 2951, 3169, 3182, 3303 3476, 3669, 3978, 4021 4045, 4183 SexAI 1 1000 SfaNI 11 218, 981, 1053, 1379 1634, 1701, 1765, 1850 2040, 3242, 3754 SfcI 8 6, 746, 2873, 3064 4102, 4111, 4158, 4195 SfiI 1 1179 SmaI 1 28 SmlI 7 293, 1802, 2684, 2961 3223, 4224, 4230 SnaBI 1 3725 SphI 3 977, 1049, 1812 SspI 2 317, 870 StuI 1 1230 StyI 5 1140, 1233, 1843, 2248 3747 TaiI 15 471, 483, 526, 636 1527, 1714, 2495, 2628 3509, 3521, 3562, 3645 3726, 3831, 4089 TaqI 16 12, 560, 1251, 1524 1680, 1704, 1740, 1902 2093, 2138, 2393, 3231 4210, 4225, 4238, 4244 TatI 4 3604, 3684, 3717, 3768 TfiI 6 1896, 2030, 2140, 2177 3355, 4069 Tru9I 17 112, 173, 294, 315 326, 338, 349, 366 464, 735, 2554, 2568 2573, 2625, 3394, 4145 4215 TseI 13 187, 706, 1371, 1472 1514, 1555, 1648, 2035 2488, 2714, 2920, 2923 2988 Tsp45I 4 701, 1532, 1838, 4086 Tsp509I 13 2, 162, 226, 304 330, 341, 988, 1060 1152, 2570, 3406, 4143 4241 TspRI 5 251, 1534, 2644, 2915 2928 Tth111I 1 1526 VspI 2 3393, 4144 XbaI 1 65 XhoI 1 4224 XhoII 9 32, 1582, 1828, 2220 2579, 2591, 2677, 2688 4220 XmaI 1 28 phCMV2/Xi Restriction Sites #sites -- Bp position of recognition site -- AatII 4 3508, 3561, 3644, 3830 Acc65I 1 17 AccI 2 11, 4192 AccIII 1 4221 AciI 61 (not expanded) AflII 1 293 AflIII 1 3329 AluI 12 189, 356, 613, 908 1196, 1516, 1974, 2771 3028, 3074, 3164, 3952 Alw44I 1 3015 AlwI 14 32, 33, 1271, 1582 1661, 1828, 2205, 2220 2579, 2592, 2677, 2689 2763, 4029 AlwNI 2 2507, 2915 ApaI 1 25 ApoI 3 225, 329, 340 AvaI 1 28 AvaII 3 1927, 2372, 4024 BamHI 1 32 BanI 6 17, 564, 1410, 1445 3852, 4186 BanII 5 25, 598, 1772, 2482 3951 BbeI 1 1410 BbsI 1 4014 BbvI 13 187, 706, 1371, 1472 1514, 1555, 1648, 2035 2488, 2714, 2920, 2923 2988 BciVI 3 829, 1619, 3120 BfaI 7 66, 260, 678, 1234 2249, 2583, 2836 BglI 5 1180, 3471, 3593, 3664 4035 BlnI 2 1233, 2248 BpmI 3 2209, 2230, 3979 BsaAI 3 525, 1713, 3725 BsaBI 2 69, 1266 BsaHI 8 1410, 2112, 2191, 3508 3561, 3644, 3830, 3984 BsaI 1 2406 BsaJI 19 21, 28, 945, 1017 1140, 1175, 1184, 1233 1574, 1843, 2248, 2375 2376, 2478, 2479, 3169 3747, 4039, 4169 BsaWI 5 1442, 2286, 2976, 3123 4221 BseMII 6 891, 1193, 2074, 2523 2646, 3055 BseRI 1 1214 BseSI 4 25, 1353, 1446, 3015 BsiEI 4 55, 1317, 2992, 4042 BsiHKAI 4 1520, 1710, 3015, 3951 BslI 20 417, 743, 1135, 1386 1930, 2211, 2243, 2244 2375, 2410, 2460, 2461 2492, 2845, 3124, 3290 3308, 3430, 4039, 4164 BsmAI 6 841, 1259, 2278, 2406 3816, 3982 BsmBI 1 3982 BsmFI 11 942, 1014, 1078, 1548 2080, 2189, 2373, 3548 3699, 3867, 4023 BsmI 2 156, 255 Bsp1286I 10 25, 598, 1353, 1446 1520, 1710, 1772, 2482 3015, 3951 BspHI 2 837, 2609 BspMI 3 1307, 1670, 2120 BsrBI 4 669, 833, 2022, 2076 BsrDI 1 1637 BsrI 8 440, 1118, 1350, 1551 2797, 2914, 2927, 3682 BssKI 22 28, 29, 39, 946 1001, 1018, 1414, 1574 1797, 2187, 2376, 2479 2951, 3169, 3182, 3303 3476, 3669, 3978, 4021 4045, 4169 BssSI 3 2003, 2129, 3156 Bst4CI 17 15, 500, 1789, 1905 2013, 2336, 2827, 3297 3368, 3461, 3583, 3654 3928, 3966, 4051, 4158 4189 BstAPI 3 972, 1044, 2506 BstBI 1 2092 BstDSI 5 21, 1140, 1843, 3747 4039 BstF5I 6 1098, 1265, 1722, 1747 2201, 3989 BstUI 18 22, 59, 334, 710 730, 754, 785, 1476 1777, 2215, 2299, 2362 2431, 2704, 3285, 3446 4040, 4065 Bsu36I 2 890, 2522 Cac8I 30 23, 628, 671, 685 958, 977, 1030, 1049 1303, 1489, 1708, 1774 1780, 1808, 1812, 1853 1857, 1911, 2194, 2381 2428, 2484, 2506, 2717 3277, 3314, 3471, 3664 4138, 4183 Cfr10I 4 628, 1730, 1911, 2194 ClaI 1 1250 Csp6I 11 18, 43, 1716, 3605 3630, 3685, 3718, 3769 3926, 4095, 4206 CviRI 20 7, 159, 186, 254 971, 980, 1043, 1052 1167, 1243, 1302, 1461 1636, 1650, 2720, 3016 3383, 4055, 4137, 4182 CviTI 76 (not expanded) DdeI 6 891, 1193, 2074, 2523 2646, 3055 DpnI 20 33, 69, 1249, 1253 1272, 1583, 1661, 1742 1751, 1829, 2205, 2221 2580, 2592, 2670, 2678 2689, 2764, 3973, 4029 DraI 3 111, 2553, 2572 DraIII 1 522 DrdI 3 476, 1432, 3221 EaeI 6 55, 1317, 1491, 1882 1909, 4042 EarI 3 883, 1760, 1970 EciI 9 895, 1092, 1113, 1123 1134, 1924, 3111, 3257 3893 Ecl136II 1 3951 Eco52I 3 55, 1317, 4042 Eco57I 3 1536, 1968, 2802 EcoO109I 1 2508 EcoRII 13 946, 1001, 1018, 1797 2376, 2479, 3169, 3182 3303, 3476, 3669, 3978 4169 EheI 1 1410 FauI 14 23, 671, 725, 1080 1101, 1541, 2078, 2216 2297, 2363, 2429, 3472 3498, 3665 Fnu4HI 31 54, 57, 187, 706 728, 742, 1180, 1319 1371, 1382, 1472, 1477 1514, 1555, 1642, 1645 1648, 1884, 1980, 2021 2035, 2149, 2488, 2503 2714, 2920, 2923, 2988 3131, 3286, 4041 FokI 6 1098, 1265, 1722, 1747 2201, 3989 FspI 1 1511 HaeII 4 674, 682, 1410, 3085 HaeIII 22 26, 56, 376, 518 1173, 1179, 1188, 1231 1318, 1492, 1883, 1910 2419, 2476, 2509, 2854 3288, 3306, 3317, 3470 3663, 4043 HgaI 7 755, 2112, 2191, 2650 3228, 3904, 3984 HhaI 20 675, 683, 709, 731 740, 753, 784, 1403 1411, 1475, 1512, 1778 2038, 2214, 2300, 2703 2812, 2986, 3086, 3153 HincII 3 11, 172, 4192 HinfI 14 62, 454, 476, 886 1896, 2030, 2082, 2140 2177, 2959, 3355, 3797 4069, 4107 HinP1I 20 675, 683, 709, 731 740, 753, 784, 1403 1411, 1475, 1512, 1778 2038, 2214, 2300, 2703 2812, 2986, 3086, 3153 HpaI 1 172 HphI 5 536, 1597, 2470, 2586 3751 KasI 1 1410 KpnI 1 17 MaeII 15 471, 483, 526, 636 1527, 1714, 2495, 2628 3509, 3521, 3562, 3645 3726, 3831, 4089 MaeIII 13 200, 701, 713, 1532 1838, 2528, 2794, 2910 2973, 3449, 3536, 3885 4086 MboII 10 655, 883, 1760, 1970 2070, 2237, 2437, 2589 2680, 4014 MnlI 26 97, 120, 129, 562 893, 1171, 1177, 1184 1190, 1214, 1217, 1229 1269, 1333, 1469, 1826 2002, 2208, 2256, 2522 2892, 3159, 3216, 3935 4006, 4036 MscI 1 1491 MslI 2 1844, 3748 MspA1I 6 21, 907, 1515, 2744 2989, 4039 MspI 21 29, 39, 629, 1316 1393, 1415, 1443, 1574 1664, 1731, 1912, 2187 2195, 2287, 2761, 2951 2977, 3124, 4021, 4045 4222 MunI 1 161 MvaI 13 946, 1001, 1018, 1797 2376, 2479, 3169, 3182 3303, 3476, 3669, 3978 4169 MwoI 30 632, 662, 689, 731 733, 972, 1044, 1095 1174, 1180, 1396, 1480 1503, 1642, 1648, 1765 1848, 2115, 2302, 2506 2705, 3277, 3471, 3593 3625, 3664, 3757, 3781 3987, 4035 NaeI 3 628, 1911, 2194 NarI 1 1410 NciI 9 28, 29, 39, 1414 1574, 2187, 2951, 4021 4045 NcoI 3 1140, 1843, 3747 NdeI 1 3620 NgoMIV 3 628, 1911, 2194 NlaIII 16 838, 978, 1050, 1141 1282, 1627, 1813, 1844 1870, 2226, 2610, 3330 3381, 3688, 3748, 4139 NlaIV 19 17, 25, 32, 564 585, 597, 788, 949 1021, 1410, 1445, 2293 2372, 2417, 3260, 3299 3852, 4023, 4186 NotI 1 54 NsiI 4 979, 1051, 3382, 4136 NspI 5 977, 1049, 1812, 3329 4138 PciI 1 3329 PleI 8 62, 454, 476, 886 2082, 2959, 3797, 4107 Ppu10I 4 979, 1051, 3382, 4136 PsiI 2 192, 397 PspOMI 1 25 PstI 2 6, 4181 PvuII 2 907, 1515 RsaI 11 18, 43, 1716, 3605 3630, 3685, 3718, 3769 3926, 4095, 4206 RsrII 1 1926 SacI 1 3951 SacII 2 21, 4039 SalI 2 11, 4192 SapI 2 1760, 1970 Sau3AI 20 33, 69, 1249, 1253 1272, 1583, 1661, 1742 1751, 1829, 2205, 2221 2580, 2592, 2670, 2678 2689, 2764, 3973, 4029 Sau96I 11 25, 26, 518, 1927 2372, 2418, 2476, 2509 3470, 3663, 4024 SchI 8 62, 454, 476, 886 2082, 2959, 3797, 4107 ScrFI 22 28, 29, 39, 946 1001, 1018, 1414, 1574 1797, 2187, 2376, 2479 2951, 3169, 3182, 3303 3476, 3669, 3978, 4021 4045, 4169 SexAI 1 1000 SfaNI 11 218, 981, 1053, 1379 1634, 1701, 1765, 1850 2040, 3242, 3754 SfcI 7 6, 746, 2873, 3064 4102, 4111, 4181 SfiI 1 1179 SmaI 1 28 SmlI 5 293, 1802, 2684, 2961 3223 SnaBI 1 3725 SphI 4 977, 1049, 1812, 4138 SspI 2 317, 870 StuI 1 1230 StyI 5 1140, 1233, 1843, 2248 3747 TaiI 15 471, 483, 526, 636 1527, 1714, 2495, 2628 3509, 3521, 3562, 3645 3726, 3831, 4089 TaqI 13 12, 560, 1251, 1524 1680, 1704, 1740, 1902 2093, 2138, 2393, 3231 4193 TatI 4 3604, 3684, 3717, 3768 TfiI 6 1896, 2030, 2140, 2177 3355, 4069 Tru9I 17 112, 173, 294, 315 326, 338, 349, 366 464, 735, 2554, 2568 2573, 2625, 3394, 4198 4233 TseI 13 187, 706, 1371, 1472 1514, 1555, 1648, 2035 2488, 2714, 2920, 2923 2988 Tsp45I 4 701, 1532, 1838, 4086 Tsp509I 11 2, 162, 226, 304 330, 341, 988, 1060 1152, 2570, 3406 TspRI 5 251, 1534, 2644, 2915 2928 Tth111I 1 1526 VspI 1 3393 XbaI 1 65 XhoII 8 32, 1582, 1828, 2220 2579, 2591, 2677, 2688 XmaI 1 28 phCMV3/Xi Restriction Sites #sites -- Bp position of recognition site -- AatII 4 3483, 3536, 3619, 3805 AccI 1 4184 AccIII 1 15 AciI 58 (not expanded) AflII 2 26, 268 AflIII 1 3304 AluI 12 164, 331, 588, 883 1171, 1491, 1949, 2746 3003, 3049, 3139, 3927 Alw44I 1 2990 AlwI 12 1246, 1557, 1636, 1803 2180, 2195, 2554, 2567 2652, 2664, 2738, 4004 AlwNI 2 2482, 2890 ApoI 3 200, 304, 315 AvaI 1 4199 AvaII 3 1902, 2347, 3999 BanI 5 539, 1385, 1420, 3827 4175 BanII 4 573, 1747, 2457, 3926 BbeI 1 1385 BbsI 1 3989 BbvI 13 162, 681, 1346, 1447 1489, 1530, 1623, 2010 2463, 2689, 2895, 2898 2963 BciVI 3 804, 1594, 3095 BfaI 7 41, 235, 653, 1209 2224, 2558, 2811 BglI 5 1155, 3446, 3568, 3639 4010 BglII 1 4195 BlnI 2 1208, 2223 BpmI 3 2184, 2205, 3954 BsaAI 3 500, 1688, 3700 BsaBI 2 44, 1241 BsaHI 8 1385, 2087, 2166, 3483 3536, 3619, 3805, 3959 BsaI 1 2381 BsaJI 17 920, 992, 1115, 1150 1159, 1208, 1549, 1818 2223, 2350, 2351, 2453 2454, 3144, 3722, 4014 4158 BsaWI 5 15, 1417, 2261, 2951 3098 BseMII 6 866, 1168, 2049, 2498 2621, 3030 BseRI 1 1189 BseSI 3 1328, 1421, 2990 BsiEI 3 1292, 2967, 4017 BsiHKAI 4 1495, 1685, 2990, 3926 BslI 20 392, 718, 1110, 1361 1905, 2186, 2218, 2219 2350, 2385, 2435, 2436 2467, 2820, 3099, 3265 3283, 3405, 4014, 4153 BsmAI 7 816, 1234, 2253, 2381 3791, 3957, 4140 BsmBI 1 3957 BsmFI 11 917, 989, 1053, 1523 2055, 2164, 2348, 3523 3674, 3842, 3998 BsmI 2 131, 230 Bsp1286I 9 573, 1328, 1421, 1495 1685, 1747, 2457, 2990 3926 BspHI 2 812, 2584 BspMI 3 1282, 1645, 2095 BsrBI 4 644, 808, 1997, 2051 BsrDI 1 1612 BsrI 8 415, 1093, 1325, 1526 2772, 2889, 2902, 3657 BssKI 19 921, 976, 993, 1389 1549, 1772, 2162, 2351 2454, 2926, 3144, 3157 3278, 3451, 3644, 3953 3996, 4020, 4158 BssSI 3 1978, 2104, 3131 Bst4CI 16 475, 1764, 1880, 1988 2311, 2802, 3272, 3343 3436, 3558, 3629, 3903 3941, 4026, 4147, 4178 BstAPI 3 947, 1019, 2481 BstBI 1 2067 BstDSI 4 1115, 1818, 3722, 4014 BstF5I 6 1073, 1240, 1697, 1722 2176, 3964 BstUI 17 34, 309, 685, 705 729, 760, 1451, 1752 2190, 2274, 2337, 2406 2679, 3260, 3421, 4015 4040 Bsu36I 2 865, 2497 Cac8I 28 603, 646, 660, 933 952, 1005, 1024, 1278 1464, 1683, 1749, 1755 1783, 1787, 1828, 1832 1886, 2169, 2356, 2403 2459, 2481, 2692, 3252 3289, 3446, 3639, 4172 Cfr10I 4 603, 1705, 1886, 2169 ClaI 1 1225 Csp6I 8 1691, 3580, 3605, 3660 3693, 3744, 3901, 4070 CviRI 19 134, 161, 229, 946 955, 1018, 1027, 1142 1218, 1277, 1436, 1611 1625, 2695, 2991, 3358 4030, 4112, 4171 CviTI 75 (not expanded) DdeI 6 866, 1168, 2049, 2498 2621, 3030 DpnI 20 44, 1224, 1228, 1247 1558, 1636, 1717, 1726 1804, 2180, 2196, 2555 2567, 2645, 2653, 2664 2739, 3948, 4004, 4196 DraI 3 86, 2528, 2547 DraIII 1 497 DrdI 3 451, 1407, 3196 EaeI 5 1292, 1466, 1857, 1884 4017 EarI 3 858, 1735, 1945 EciI 9 870, 1067, 1088, 1098 1109, 1899, 3086, 3232 3868 Ecl136II 1 3926 Eco52I 2 1292, 4017 Eco57I 3 1511, 1943, 2777 EcoO109I 1 2483 EcoRII 13 921, 976, 993, 1772 2351, 2454, 3144, 3157 3278, 3451, 3644, 3953 4158 EheI 1 1385 FauI 13 646, 700, 1055, 1076 1516, 2053, 2191, 2272 2338, 2404, 3447, 3473 3640 Fnu4HI 30 32, 162, 681, 703 717, 1155, 1294, 1346 1357, 1447, 1452, 1489 1530, 1617, 1620, 1623 1859, 1955, 1996, 2010 2124, 2463, 2478, 2689 2895, 2898, 2963, 3106 3261, 4016 FokI 6 1073, 1240, 1697, 1722 2176, 3964 FspI 1 1486 HaeII 4 649, 657, 1385, 3060 HaeIII 21 31, 351, 493, 1148 1154, 1163, 1206, 1293 1467, 1858, 1885, 2394 2451, 2484, 2829, 3263 3281, 3292, 3445, 3638 4018 HgaI 7 730, 2087, 2166, 2625 3203, 3879, 3959 HhaI 20 650, 658, 684, 706 715, 728, 759, 1378 1386, 1450, 1487, 1753 2013, 2189, 2275, 2678 2787, 2961, 3061, 3128 HincII 2 147, 4184 HinfI 15 37, 429, 451, 861 1871, 2005, 2057, 2115 2152, 2934, 3330, 3772 4044, 4082, 4127 HinP1I 20 650, 658, 684, 706 715, 728, 759, 1378 1386, 1450, 1487, 1753 2013, 2189, 2275, 2678 2787, 2961, 3061, 3128 HpaI 1 147 HphI 5 511, 1572, 2445, 2561 3726 KasI 1 1385 MaeII 15 446, 458, 501, 611 1502, 1689, 2470, 2603 3484, 3496, 3537, 3620 3701, 3806, 4064 MaeIII 14 175, 676, 688, 1507 1813, 2503, 2769, 2885 2948, 3424, 3511, 3860 4061, 4204 MboII 10 630, 858, 1735, 1945 2045, 2212, 2412, 2564 2655, 3989 MnlI 26 72, 95, 104, 537 868, 1146, 1152, 1159 1165, 1189, 1192, 1204 1244, 1308, 1444, 1801 1977, 2183, 2231, 2497 2867, 3134, 3191, 3910 3981, 4011 MscI 1 1466 MslI 2 1819, 3723 MspA1I 5 882, 1490, 2719, 2964 4014 MspI 19 16, 604, 1291, 1368 1390, 1418, 1549, 1639 1706, 1887, 2162, 2170 2262, 2736, 2926, 2952 3099, 3996, 4020 MunI 1 136 MvaI 13 921, 976, 993, 1772 2351, 2454, 3144, 3157 3278, 3451, 3644, 3953 4158 MwoI 30 607, 637, 664, 706 708, 947, 1019, 1070 1149, 1155, 1371, 1455 1478, 1617, 1623, 1740 1823, 2090, 2277, 2481 2680, 3252, 3446, 3568 3600, 3639, 3732, 3756 3962, 4010 NaeI 3 603, 1886, 2169 NarI 1 1385 NciI 6 1389, 1549, 2162, 2926 3996, 4020 NcoI 3 1115, 1818, 3722 NdeI 1 3595 NgoMIV 3 603, 1886, 2169 NlaIII 16 813, 953, 1025, 1116 1257, 1602, 1788, 1819 1845, 2201, 2585, 3305 3356, 3663, 3723, 4114 NlaIV 16 539, 560, 572, 763 924, 996, 1385, 1420 2268, 2347, 2392, 3235 3274, 3827, 3998, 4175 NsiI 4 954, 1026, 3357, 4111 NspI 4 952, 1024, 1787, 3304 PciI 1 3304 PleI 9 37, 429, 451, 861 2057, 2934, 3772, 4082 4127 Ppu10I 4 954, 1026, 3357, 4111 PsiI 2 167, 372 PstI 1 4170 PvuII 2 882, 1490 RsaI 8 1691, 3580, 3605, 3660 3693, 3744, 3901, 4070 RsrII 1 1901 SacI 1 3926 SacII 1 4014 SalI 1 4184 SapI 2 1735, 1945 Sau3AI 20 44, 1224, 1228, 1247 1558, 1636, 1717, 1726 1804, 2180, 2196, 2555 2567, 2645, 2653, 2664 2739, 3948, 4004, 4196 Sau96I 9 493, 1902, 2347, 2393 2451, 2484, 3445, 3638 3999 SchI 9 37, 429, 451, 861 2057, 2934, 3772, 4082 4127 ScrFI 19 921, 976, 993, 1389 1549, 1772, 2162, 2351 2454, 2926, 3144, 3157 3278, 3451, 3644, 3953 3996, 4020, 4158 SexAI 1 975 SfaNI 11 193, 956, 1028, 1354 1609, 1676, 1740, 1825 2015, 3217, 3729 SfcI 7 721, 2848, 3039, 4077 4086, 4133, 4170 SfiI 1 1154 SmlI 7 26, 268, 1777, 2659 2936, 3198, 4199 SnaBI 1 3700 SphI 3 952, 1024, 1787 SspI 2 292, 845 StuI 1 1205 StyI 5 1115, 1208, 1818, 2223 3722 TaiI 15 446, 458, 501, 611 1502, 1689, 2470, 2603 3484, 3496, 3537, 3620 3701, 3806, 4064 TaqI 13 535, 1226, 1499, 1655 1679, 1715, 1877, 2068 2113, 2368, 3206, 4185 4200 TatI 4 3579, 3659, 3692, 3743 TfiI 6 1871, 2005, 2115, 2152 3330, 4044 Tru9I 18 27, 87, 148, 269 290, 301, 313, 324 341, 439, 710, 2529 2543, 2548, 2600, 3369 4120, 4190 TseI 13 162, 681, 1346, 1447 1489, 1530, 1623, 2010 2463, 2689, 2895, 2898 2963 Tsp45I 4 676, 1507, 1813, 4061 Tsp509I 11 137, 201, 279, 305 316, 963, 1035, 1127 2545, 3381, 4118 TspRI 5 226, 1509, 2619, 2890 2903 Tth111I 1 1501 VspI 2 3368, 4119 XbaI 1 40 XhoI 1 4199 XhoII 8 1557, 1803, 2195, 2554 2566, 2652, 2663, 4195 pIX/Xi – Restriction Sites #sites -- Bp position of recognition site -- AatII 1 2696 AccIII 1 128 AciI 29 48, 138, 280, 336, 379, 386, 407, 498 526, 653, 672, 793, 903, 1038, 1047, 1583 2421, 2619, 2666, 2840, 2879, 2889, 2915, 2953 2966, 2992, 3022, 3061, 3099 AflIII 1 453 AluI 11 28, 289, 304, 394, 620, 756, 1013, 1457 1907, 2808, 2827 Alw44I 2 767, 2942 AlwI 10 1020, 1094, 1106, 1191, 1204, 1543, 1776, 2157 3050, 3057 AlwNI 1 864 ApoI 3 319, 2227, 2411 AvaI 2 2152, 2426 AvaII 1 1472 BanI 3 198, 241, 1294 BanII 1 2373 BbvCI 1 1360 BbvI 10 5, 38, 41, 358, 376, 795, 860, 863 1069, 2806 BciVI 2 662, 2621 BfaI 5 62, 948, 1201, 3047, 3117 Bpu10I 2 1360, 2007 Bpu1102I 1 50 BsaBI 1 3051 BsaHI 1 2696 BsaJI 10 73, 188, 202, 613, 1345, 1443, 1504, 1751 2152, 2153 BsaWI 4 128, 659, 806, 1889 BseMII 9 30, 51, 728, 1137, 1303, 1361, 1553, 2008 2936 BseSI 2 767, 2942 BsiEI 3 366, 790, 2027 BsiHKAI 2 767, 2942 BslI 13 81, 137, 469, 487, 653, 932, 1338, 1345 1504, 1762, 1779, 2111, 2812 BsmAI 5 2014, 2553, 2610, 2763, 2816 BsmBI 3 2014, 2762, 2816 BsmFI 1 1588 BsmI 2 2063, 2140 Bsp1286I 3 767, 2373, 2942 BspHI 4 1173, 2506, 2613, 2718 BsrBI 3 384, 2617, 3097 BsrDI 1 2531 BsrI 6 856, 869, 986, 1475, 1615, 2239 BssKI 14 148, 188, 480, 601, 614, 832, 1345, 1400 1779, 2136, 2152, 2153, 2812, 2847 BssSI 3 626, 1372, 2749 Bst4CI 14 196, 239, 266, 415, 486, 956, 1269, 1445 1511, 1984, 2476, 2493, 2784, 2819 BstAPI 1 2944 BstDSI 2 1443, 1504 BstF5I 7 185, 206, 1326, 1420, 1767, 2400, 2842 BstUI 10 499, 1080, 2025, 2370, 2420, 2667, 2767, 2769 2872, 3062 Cac8I 8 382, 468, 505, 1065, 1941, 2087, 2371, 2901 Cfr10I 2 157, 2070 ClaI 2 294, 2334 Csp6I 3 155, 2192, 2933 CviRI 10 325, 768, 1064, 1676, 1913, 2066, 2179, 2530 2805, 2943 CviTI 46 4, 16, 28, 37, 79, 146, 170, 289 304, 375, 394, 467, 478, 496, 522, 620 756, 761, 786, 865, 930, 941, 984, 1013 1403, 1425, 1441, 1457, 1581, 1721, 1907, 1940 1944, 2237, 2321, 2374, 2423, 2454, 2565, 2754 2808, 2827, 2860, 2900, 2904, 3004 DdeI 10 30, 51, 728, 1137, 1303, 1361, 1553, 2008 2701, 2936 DpnI 14 1020, 1095, 1106, 1114, 1192, 1204, 1309, 1538 1543, 1776, 2028, 2158, 3051, 3057 DraI 2 1210, 1229 DraIII 1 2557 DrdI 2 555, 2856 EarI 1 2217 EciI 3 525, 671, 3021 Eco57I 1 980 EcoNI 1 2111 EcoO109I 2 77, 2753 EcoRI 1 319 EcoRII 7 188, 480, 601, 614, 1400, 1779, 2136 EcoRV 2 132, 3017 FauI 4 137, 2879, 2889, 3060 Fnu4HI 16 5, 38, 41, 358, 376, 379, 497, 652 795, 860, 863, 1069, 1582, 2421, 2806, 2915 FokI 7 185, 206, 1326, 1420, 1767, 2400, 2842 HaeII 2 697, 1357 HaeIII 10 79, 146, 467, 478, 496, 930, 1403, 1940 2423, 2754 HgaI 3 555, 1133, 2873 HhaI 15 218, 361, 631, 698, 798, 972, 1081, 1358 2004, 2074, 2091, 2313, 2668, 2768, 2871 HincII 1 3029 HindIII 2 288, 1906 HinfI 13 329, 353, 428, 824, 1341, 1392, 1408, 1795 1801, 1886, 2058, 2114, 3076 HinP1I 15 218, 361, 631, 698, 798, 972, 1081, 1358 2004, 2074, 2091, 2313, 2668, 2768, 2871 HphI 10 192, 1197, 1481, 1748, 1868, 1892, 2108, 2167 2777, 2786 MaeII 4 1156, 2436, 2561, 2697 MaeIII 11 193, 810, 873, 989, 1272, 1620, 1877, 2172 2264, 2536, 2822 MboII 7 332, 1103, 1194, 1377, 1528, 2126, 2217 MnlI 20 81, 97, 117, 144, 204, 342, 568, 625 892, 1292, 1363, 1371, 1436, 1753, 1836, 2220 2425, 2752, 2794, 3114 MspA1I 4 48, 793, 1038, 2877 MspI 14 2, 129, 149, 158, 660, 807, 833, 1023 1345, 1890, 2071, 2153, 2813, 2847 MvaI 7 188, 480, 601, 614, 1400, 1779, 2136 MwoI 13 8, 29, 41, 152, 386, 500, 1072, 1404 2067, 2281, 2313, 2944, 3015 NciI 7 148, 832, 1345, 2152, 2153, 2812, 2847 NdeI 1 2948 NlaIII 13 314, 454, 1174, 1872, 2177, 2292, 2404, 2507 2614, 2719, 2803, 3026, 3156 NlaIV 8 77, 198, 241, 483, 522, 1294, 1439, 2662 NruI 1 2369 NsiI 2 1912, 2178 NspI 2 453, 2802 PciI 1 453 PleI 6 353, 824, 1341, 1392, 1801, 3076 Ppu10I 2 1912, 2178 PstI 1 324 PvuI 1 2027 RsaI 3 155, 2192, 2933 Sau3AI 14 1020, 1095, 1106, 1114, 1192, 1204, 1309, 1538 1543, 1776, 2028, 2158, 3051, 3057 Sau96I 4 78, 146, 1472, 2754 SchI 6 353, 824, 1341, 1392, 1801, 3076 ScrFI 14 148, 188, 480, 601, 614, 832, 1345, 1400 1779, 2136, 2152, 2153, 2812, 2847 SfaNI 13 184, 541, 1531, 1662, 1973, 2093, 2180, 2315 2399, 2843, 2918, 2973, 2994 SfcI 5 179, 324, 718, 909, 3082 SgfI 1 2026 SmaI 1 2152 SmlI 5 94, 559, 821, 1098, 2426 SspI 1 2101 StuI 1 1402 StyI 1 73 TaiI 4 1156, 2436, 2561, 2697 TaqI 8 295, 553, 1565, 1658, 2061, 2335, 2427, 3055 TatI 1 2932 TfiI 7 329, 428, 1408, 1795, 1886, 2058, 2114 Tru9I 16 263, 285, 1159, 1211, 1216, 1230, 1283, 1631 1827, 2036, 2415, 2726, 2907, 3069, 3133, 3139 TseI 10 5, 38, 41, 358, 376, 795, 860, 863 1069, 2806 Tsp45I 3 193, 1877, 2822 Tsp509I 14 260, 320, 1214, 1629, 1637, 1679, 1829, 2046 2228, 2412, 3067, 3091, 3108, 3125 TspRI 8 349, 855, 868, 1139, 1288, 1616, 2083, 2163 Van91I 1 1762 VspI 2 1826, 3068 XbaI 1 3116 XhoI 1 2426 XhoII 6 1094, 1105, 1191, 1203, 1775, 3050 XmaI 1 2152 pIX2/Xi Restriction Sites #sites -- Bp position of recognition site -- AatII 1 2696 AccIII 1 128 AciI 29 48, 138, 280, 336 379, 386, 407, 498 526, 653, 672, 793 903, 1038, 1047, 1583 2421, 2619, 2666, 2840 2879, 2889, 2915, 2953 2966, 2992, 3022, 3061 3099 AflIII 1 453 AluI 11 28, 289, 304, 394 620, 756, 1013, 1457 1907, 2808, 2827 Alw44I 2 767, 2942 AlwI 10 1020, 1094, 1106, 1191 1204, 1543, 1776, 2157 3050, 3057 AlwNI 1 864 ApoI 3 319, 2227, 2411 AvaI 2 2152, 2426 AvaII 1 1472 BanI 3 198, 241, 1294 BanII 1 2373 BbvCI 1 1360 BbvI 10 5, 38, 41, 358 376, 795, 860, 863 1069, 2806 BciVI 2 662, 2621 BfaI 5 62, 948, 1201, 3047 3117 Bpu10I 2 1360, 2007 Bpu1102I 1 50 BsaBI 1 3051 BsaHI 1 2696 BsaJI 10 73, 188, 202, 613 1345, 1443, 1504, 1751 2152, 2153 BsaWI 4 128, 659, 806, 1889 BseMII 9 30, 51, 728, 1137 1303, 1361, 1553, 2008 2936 BseSI 2 767, 2942 BsiEI 3 366, 790, 2027 BsiHKAI 2 767, 2942 BslI 13 81, 137, 469, 487 653, 932, 1338, 1345 1504, 1762, 1779, 2111 2812 BsmAI 5 2014, 2553, 2610, 2763 2816 BsmBI 3 2014, 2762, 2816 BsmFI 1 1588 BsmI 2 2063, 2140 Bsp1286I 3 767, 2373, 2942 BspHI 4 1173, 2506, 2613, 2718 BsrBI 3 384, 2617, 3097 BsrDI 1 2531 BsrI 6 856, 869, 986, 1475 1615, 2239 BssKI 14 148, 188, 480, 601 614, 832, 1345, 1400 1779, 2136, 2152, 2153 2812, 2847 BssSI 3 626, 1372, 2749 Bst4CI 14 196, 239, 266, 415 486, 956, 1269, 1445 1511, 1984, 2476, 2493 2784, 2819 BstAPI 1 2944 BstDSI 2 1443, 1504 BstF5I 7 185, 206, 1326, 1420 1767, 2400, 2842 BstUI 10 499, 1080, 2025, 2370 2420, 2667, 2767, 2769 2872, 3062 Cac8I 8 382, 468, 505, 1065 1941, 2087, 2371, 2901 Cfr10I 2 157, 2070 ClaI 2 294, 2334 Csp6I 3 155, 2192, 2933 CviRI 11 325, 768, 1064, 1676 1913, 2066, 2179, 2530 2805, 2943, 3158 CviTI 46 4, 16, 28, 37 79, 146, 170, 289 304, 375, 394, 467 478, 496, 522, 620 756, 761, 786, 865 930, 941, 984, 1013 1403, 1425, 1441, 1457 1581, 1721, 1907, 1940 1944, 2237, 2321, 2374 2423, 2454, 2565, 2754 2808, 2827, 2860, 2900 2904, 3004 DdeI 10 30, 51, 728, 1137 1303, 1361, 1553, 2008 2701, 2936 DpnI 14 1020, 1095, 1106, 1114 1192, 1204, 1309, 1538 1543, 1776, 2028, 2158 3051, 3057 DraI 2 1210, 1229 DraIII 1 2557 DrdI 2 555, 2856 EarI 1 2217 EciI 3 525, 671, 3021 Eco57I 1 980 EcoNI 1 2111 EcoO109I 2 77, 2753 EcoRI 1 319 EcoRII 7 188, 480, 601, 614 1400, 1779, 2136 EcoRV 2 132, 3017 FauI 4 137, 2879, 2889, 3060 Fnu4HI 16 5, 38, 41, 358 376, 379, 497, 652 795, 860, 863, 1069 1582, 2421, 2806, 2915 FokI 7 185, 206, 1326, 1420 1767, 2400, 2842 HaeII 2 697, 1357 HaeIII 10 79, 146, 467, 478 496, 930, 1403, 1940 2423, 2754 HgaI 3 555, 1133, 2873 HhaI 15 218, 361, 631, 698 798, 972, 1081, 1358 2004, 2074, 2091, 2313 2668, 2768, 2871 HincII 1 3029 HindIII 2 288, 1906 HinfI 13 329, 353, 428, 824 1341, 1392, 1408, 1795 1801, 1886, 2058, 2114 3076 HinP1I 15 218, 361, 631, 698 798, 972, 1081, 1358 2004, 2074, 2091, 2313 2668, 2768, 2871 HphI 11 192, 1197, 1481, 1748 1868, 1892, 2108, 2167 2777, 2786, 3165 MaeII 4 1156, 2436, 2561, 2697 MaeIII 11 193, 810, 873, 989 1272, 1620, 1877, 2172 2264, 2536, 2822 MboII 7 332, 1103, 1194, 1377 1528, 2126, 2217 MnlI 20 81, 97, 117, 144 204, 342, 568, 625 892, 1292, 1363, 1371 1436, 1753, 1836, 2220 2425, 2752, 2794, 3114 MspA1I 4 48, 793, 1038, 2877 MspI 14 2, 129, 149, 158 660, 807, 833, 1023 1345, 1890, 2071, 2153 2813, 2847 MvaI 7 188, 480, 601, 614 1400, 1779, 2136 MwoI 13 8, 29, 41, 152 386, 500, 1072, 1404 2067, 2281, 2313, 2944 3015 NciI 7 148, 832, 1345, 2152 2153, 2812, 2847 NdeI 1 2948 NlaIII 13 314, 454, 1174, 1872 2177, 2292, 2404, 2507 2614, 2719, 2803, 3026 3156 NlaIV 8 77, 198, 241, 483 522, 1294, 1439, 2662 NruI 1 2369 NsiI 3 1912, 2178, 3157 NspI 2 453, 2802 PciI 1 453 PleI 6 353, 824, 1341, 1392 1801, 3076 Ppu10I 3 1912, 2178, 3157 PstI 1 324 PvuI 1 2027 RsaI 3 155, 2192, 2933 Sau3AI 14 1020, 1095, 1106, 1114 1192, 1204, 1309, 1538 1543, 1776, 2028, 2158 3051, 3057 Sau96I 4 78, 146, 1472, 2754 SchI 6 353, 824, 1341, 1392 1801, 3076 ScrFI 14 148, 188, 480, 601 614, 832, 1345, 1400 1779, 2136, 2152, 2153 2812, 2847 SfaNI 14 184, 541, 1531, 1662 1973, 2093, 2180, 2315 2399, 2843, 2918, 2973 2994, 3159 SfcI 5 179, 324, 718, 909 3082 SgfI 1 2026 SmaI 1 2152 SmlI 5 94, 559, 821, 1098 2426 SspI 1 2101 StuI 1 1402 StyI 1 73 TaiI 4 1156, 2436, 2561, 2697 TaqI 8 295, 553, 1565, 1658 2061, 2335, 2427, 3055 TatI 1 2932 TfiI 7 329, 428, 1408, 1795 1886, 2058, 2114 Tru9I 16 263, 285, 1159, 1211 1216, 1230, 1283, 1631 1827, 2036, 2415, 2726 2907, 3069, 3133, 3139 TseI 10 5, 38, 41, 358 376, 795, 860, 863 1069, 2806 Tsp45I 3 193, 1877, 2822 Tsp509I 14 260, 320, 1214, 1629 1637, 1679, 1829, 2046 2228, 2412, 3067, 3091 3108, 3125 TspRI 8 349, 855, 868, 1139 1288, 1616, 2083, 2163 Van91I 1 1762 VspI 2 1826, 3068 XbaI 1 3116 XhoI 1 2426 XhoII 6 1094, 1105, 1191, 1203 1775, 3050 XmaI 1 2152 pIX3/Xi Restriction Sites #sites -- Bp position of recognition site -- AatII 1 2728 AccIII 2 15, 160 AciI 29 80, 170, 312, 368 411, 418, 439, 530 558, 685, 704, 825 935, 1070, 1079, 1615 2453, 2651, 2698, 2872 2911, 2921, 2947, 2985 2998, 3024, 3054, 3093 3131 AflII 1 26 AflIII 1 485 AluI 11 60, 321, 336, 426 652, 788, 1045, 1489 1939, 2840, 2859 Alw44I 2 799, 2974 AlwI 11 31, 1052, 1126, 1138 1223, 1236, 1575, 1808 2189, 3082, 3089 AlwNI 1 896 ApoI 3 351, 2259, 2443 AvaI 2 2184, 2458 AvaII 1 1504 BanI 3 230, 273, 1326 BanII 1 2405 BbvCI 1 1392 BbvI 10 37, 70, 73, 390 408, 827, 892, 895 1101, 2838 BciVI 2 694, 2653 BfaI 5 94, 980, 1233, 3079 3149 Bpu10I 2 1392, 2039 Bpu1102I 1 82 BsaBI 1 3083 BsaHI 1 2728 BsaJI 10 105, 220, 234, 645 1377, 1475, 1536, 1783 2184, 2185 BsaWI 5 15, 160, 691, 838 1921 BseMII 9 62, 83, 760, 1169 1335, 1393, 1585, 2040 2968 BseSI 2 799, 2974 BsiEI 3 398, 822, 2059 BsiHKAI 2 799, 2974 BslI 13 113, 169, 501, 519 685, 964, 1370, 1377 1536, 1794, 1811, 2143 2844 BsmAI 5 2046, 2585, 2642, 2795 2848 BsmBI 3 2046, 2794, 2848 BsmFI 1 1620 BsmI 2 2095, 2172 Bsp1286I 3 799, 2405, 2974 BspHI 4 1205, 2538, 2645, 2750 BsrBI 3 416, 2649, 3129 BsrDI 1 2563 BsrI 6 888, 901, 1018, 1507 1647, 2271 BssKI 14 180, 220, 512, 633 646, 864, 1377, 1432 1811, 2168, 2184, 2185 2844, 2879 BssSI 3 658, 1404, 2781 Bst4CI 14 228, 271, 298, 447 518, 988, 1301, 1477 1543, 2016, 2508, 2525 2816, 2851 BstAPI 1 2976 BstDSI 2 1475, 1536 BstF5I 7 217, 238, 1358, 1452 1799, 2432, 2874 BstUI 10 531, 1112, 2057, 2402 2452, 2699, 2799, 2801 2904, 3094 Cac8I 8 414, 500, 537, 1097 1973, 2119, 2403, 2933 Cfr10I 2 189, 2102 ClaI 2 326, 2366 Csp6I 4 187, 2224, 2965, 3191 CviRI 10 357, 800, 1096, 1708 1945, 2098, 2211, 2562 2837, 2975 CviTI 46 36, 48, 60, 69 111, 178, 202, 321 336, 407, 426, 499 510, 528, 554, 652 788, 793, 818, 897 962, 973, 1016, 1045 1435, 1457, 1473, 1489 1613, 1753, 1939, 1972 1976, 2269, 2353, 2406 2455, 2486, 2597, 2786 2840, 2859, 2892, 2932 2936, 3036 DdeI 10 62, 83, 760, 1169 1335, 1393, 1585, 2040 2733, 2968 DpnI 15 31, 1052, 1127, 1138 1146, 1224, 1236, 1341 1570, 1575, 1808, 2060 2190, 3083, 3089 DraI 2 1242, 1261 DraIII 1 2589 DrdI 2 587, 2888 EarI 1 2249 EciI 3 557, 703, 3053 Eco57I 1 1012 EcoNI 1 2143 EcoO109I 2 109, 2785 EcoRI 1 351 EcoRII 7 220, 512, 633, 646 1432, 1811, 2168 EcoRV 2 164, 3049 FauI 4 169, 2911, 2921, 3092 Fnu4HI 16 37, 70, 73, 390 408, 411, 529, 684 827, 892, 895, 1101 1614, 2453, 2838, 2947 FokI 7 217, 238, 1358, 1452 1799, 2432, 2874 HaeII 2 729, 1389 HaeIII 10 111, 178, 499, 510 528, 962, 1435, 1972 2455, 2786 HgaI 3 587, 1165, 2905 HhaI 15 250, 393, 663, 730 830, 1004, 1113, 1390 2036, 2106, 2123, 2345 2700, 2800, 2903 HincII 1 3061 HindIII 2 320, 1938 HinfI 13 361, 385, 460, 856 1373, 1424, 1440, 1827 1833, 1918, 2090, 2146 3108 HinP1I 15 250, 393, 663, 730 830, 1004, 1113, 1390 2036, 2106, 2123, 2345 2700, 2800, 2903 HphI 10 224, 1229, 1513, 1780 1900, 1924, 2140, 2199 2809, 2818 MaeII 4 1188, 2468, 2593, 2729 MaeIII 11 225, 842, 905, 1021 1304, 1652, 1909, 2204 2296, 2568, 2854 MboII 7 364, 1135, 1226, 1409 1560, 2158, 2249 MnlI 20 113, 129, 149, 176 236, 374, 600, 657 924, 1324, 1395, 1403 1468, 1785, 1868, 2252 2457, 2784, 2826, 3146 MspA1I 4 80, 825, 1070, 2909 MspI 15 16, 34, 161, 181 190, 692, 839, 865 1055, 1377, 1922, 2103 2185, 2845, 2879 MvaI 7 220, 512, 633, 646 1432, 1811, 2168 MwoI 13 40, 61, 73, 184 418, 532, 1104, 1436 2099, 2313, 2345, 2976 3047 NciI 7 180, 864, 1377, 2184 2185, 2844, 2879 NdeI 1 2980 NlaIII 13 346, 486, 1206, 1904 2209, 2324, 2436, 2539 2646, 2751, 2835, 3058 3188 NlaIV 8 109, 230, 273, 515 554, 1326, 1471, 2694 NruI 1 2401 NsiI 2 1944, 2210 NspI 2 485, 2834 PciI 1 485 PleI 6 385, 856, 1373, 1424 1833, 3108 Ppu10I 2 1944, 2210 PstI 1 356 PvuI 1 2059 RsaI 4 187, 2224, 2965, 3191 Sau3AI 15 31, 1052, 1127, 1138 1146, 1224, 1236, 1341 1570, 1575, 1808, 2060 2190, 3083, 3089 Sau96I 4 110, 178, 1504, 2786 SchI 6 385, 856, 1373, 1424 1833, 3108 ScrFI 14 180, 220, 512, 633 646, 864, 1377, 1432 1811, 2168, 2184, 2185 2844, 2879 SfaNI 13 216, 573, 1563, 1694 2005, 2125, 2212, 2347 2431, 2875, 2950, 3005 3026 SfcI 5 211, 356, 750, 941 3114 SgfI 1 2058 SmaI 1 2184 SmlI 6 26, 126, 591, 853 1130, 2458 SspI 1 2133 StuI 1 1434 StyI 1 105 TaiI 4 1188, 2468, 2593, 2729 TaqI 8 327, 585, 1597, 1690 2093, 2367, 2459, 3087 TatI 1 2964 TfiI 7 361, 460, 1440, 1827 1918, 2090, 2146 Tru9I 17 27, 295, 317, 1191 1243, 1248, 1262, 1315 1663, 1859, 2068, 2447 2758, 2939, 3101, 3165 3171 TseI 10 37, 70, 73, 390 408, 827, 892, 895 1101, 2838 Tsp45I 3 225, 1909, 2854 Tsp509I 14 292, 352, 1246, 1661 1669, 1711, 1861, 2078 2260, 2444, 3099, 3123 3140, 3157 TspRI 8 381, 887, 900, 1171 1320, 1648, 2115, 2195 Van91I 1 1794 VspI 2 1858, 3100 XbaI 1 3148 XhoI 1 2458 XhoII 7 30, 1126, 1137, 1223 1235, 1807, 3082 XmaI 1 2184