Description:
The phCMV Fusion Stable Reporter (FSR) Vectors
are designed for high-level expression of GFP or Luciferase fusion proteins
and for creating GFP or Luciferase stable cell lines. These innovative vectors
contain optimized CMV promoter-intron sequences for significantly higher constitutive
expression levels than other mammalian expression vectors. Each phCMV-FSR Vector
contains a multiple cloning site on either side of the reporter gene for creating
N- or C-terminal fusion proteins. Also, each vector contains a combination Kanamycin/Neomycin
antibiotic selection gene, which allows for selection in both E. coli
and mammalian cells, while minimizing vector size so that transfection efficiency
is maximally enhanced. The vectors can be used for either transient transfection
or for generating stable cells lines expressing the reporter gene only or your
fusion-reporter gene construct.
The phCMV-FSR Vectors provide the following features and benefits:
| Feature |
Benefit |
| Modified hCMV immediate-early promoter |
High level constitutive expression
pomoter in a wide variety of cells and cell types. |
| Reporter genes |
Convenient choice of GFP or Luciferase
reporter genes for control expression experiments. |
| SV40 polyadenylation signal |
Stable mRNA by providing efficient
transcription termination and polyadenylation. |
| Kanamycin/Neomycin resistance gene |
Efficient vector selection in E.
coli (with Kanamycin sulfate) or mammalian cells (with Neomycin
Sulfate) |
| PUC Origin of replication |
High copy number replication of vector
in E. coli. |
| Two multiple cloning sites (MCS) |
For cloning gene of choice upstream
or downstream of reporter gene (in fusion). |
Storage:
Store at -20°C; stable for 1 year
