| Description
GeneSilencer® siRNA Transfection Reagent is a novel
cationic lipid formulation specifically designed for efficient delivery
of siRNAs (small interfering RNAs) into a wide variety of cell types.
siRNAs are short, gene-specific double-stranded RNAs that can cause gene
silencing in mammalian cells by catalytically cleaving greater than 95%
of the target mRNA (1,2,3).
Contents
– Hydrated GeneSilencer® Lipid (1 vial
x 750 µl for T500750 or 5 vials x 750 µl for T505750)
– siRNA Diluent (1 tube x 4 ml for T500750 / 5 tubes x 4 ml
for T505750)
Storage
The GeneSilencer® siRNA Transfection Reagent is shipped
at room temperature. For maximum stability, store all reagents at 4ºC
upon receipt. If stored properly, all components are stable for 6 months.
References
1. Elbashir, S.M. et al. (2001) Nature 411:
494-498.
2. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98:
9742-9747.
3. Sharp, P.A. (2001) Genes and Development 15:
485-490.
|
293 cells transiently transfected with 1 µg of gWiz/GFP. When they
are co-transfected with 500 ng of GFP siRNA, GFP expression was efficiently
suppressed. (GFP: green, left panels. DAPI: blue, right panels) |
