SoluLyse-M™ Mammalian Protein Extraction Reagent provides a highly efficient
yet gentle method for extracting proteins from mammalian cells. It extracts
cytoplasmic and nuclear protein from cultured cell lines using a proprietary
non-ionic detergent in 25mM Tris, pH 7.4 and 250mM Sucrose. Lysis with SoluLyse-M™
reagent is so efficient that adherent cells do not need to be scraped from the
culture dish or multi-well plates. Total protein can be recovered by simply
adding an appropriate amount of SoluLyse-M reagent into the container and shaking
for 10 minutes. This is especially important when the cells are grown in 96-wll
or 24-well plates.
SoluLyse-M™ Mammalian Protein Extraction Reagent is compatible with many
different applications, such as reporter assays, immunoassays and protein purification.
The reagent is easily dialyzable and compatible with protein assays such as
Western blotting, Coomassie Blue, Bradford and the BCA™ Protein Assays.
In addition, the reagent is compatible with a variety of additives such as protease
inhibitors and reducing agents and may be readily dialyzed if needed.
 |
| Compatibility with Protein Purification. SoluLyse-M reagent
was used to lyse CHO-K1 cells expressing 6xHis-LacZ. The lysate was purified
with Ni-NTA resin. Western blotting was performed using anti-His antibody. |
Contents
- SoluLyse-M Protein Extraction Reagent is supplied in 1X solution in
25mM
Tris, pH 7.4 and 250mM Sucrose.
Storage
Store at room temperature or +4°C upon receipt. Stable for 2 years when stored properly.
