Side-by-side comparisons between SoluLyze and another leading commercial
lysis reagent showed that SoluLyse yields about 10-fold more soluble proteins.
In addition, SoluLyse does not compromise protein activities in cell lysates.
The SoluLyse reagent is easy to use. Simply add it to a bacterial pellet and
gently mix for 10 minutes. The cells will be disrupted and soluble proteins
released. Insoluble cell debris can then be removed by centrifugation. SoluLyse
is also useful for the preparation of high-purity inclusion bodies when the
protein of interest is insoluble.
The SoluLyse Reagent is tested to be compatible with all popular purification
methods, such as Ni and glutathione resins for purifying poly-His and GST-tagged
proteins. The unique, mild formulation of SoluLyse offers minimized interference
with the binding and elution processes and ensures superior purification results.
In addition, the reagent is compatible with a variety of additives such as protease
inhibitors and reducing agents and may be readily dialyzed if needed.
Contents
- SoluLyse Protein Extraction Reagent is supplied in 1X solution in
50mM
NaHPO4, pH 7.4.
- SoluLyse Reagent in Tris Buffer is supplied in 1X solution in 20 mM Tris·HCl, pH 7.5.
Storage
Store at room temperature or +4°C upon receipt. Stable for 2 years when stored properly.
