Description
The Recombinant Human Turbo Dicer Enzyme Kit uses an ultra-active form of human recombinant dicer enzyme that can cleave more than 95% of dsRNA template into 22 bp siRNAs within 2 hours under optimized reaction conditions. In contrast to individual synthetic siRNAs, the Turbo Dicer kit allows you to quickly produce multiple siRNA species against your target mRNA, and thereby achieve effective gene silencing with minimal guesswork.
Figure 1. Turbo Dicer Digestion of dsRNA
Lane 1: 100 bp marker. Lane 2: 700bp dsRNA, 1µg undigested. Lane 3: 700 bp dsRNA, 1µg digested 15 hours with 1 unit of Dicer Enzyme. Lane 4: 700 bp dsRNA, 1µg digested 2 hours with 1 unit of Turbo Dicer Enzyme.
For maximum convenience, the Turbo Dicer siRNA Generation Kit contains everything that is required for preparing double stranded RNA, RNA cleavage, siRNA cleanup and transfection. Both the Recombinant Turbo Dicer Enzyme and the RNA Purification Columns are also available separately.
Shipping and Storage: The Recombinant Human Turbo Dicer Enzyme Kit is shipped frozen. For maximum stability and long-term use, immediately store at -20oC upon receipt. All components are stable for six months when stored properly.
